Journal: Polymers

Article Title: Antibacterial Chitosan Nanofiber Thin Films with Bacitracin Zinc Salt

doi: 10.3390/polym13071104

Figure Lengend Snippet: The cell viability of L929 fibroblasts after incubation with the thin films for 24 h (** p < 0.01).

Article Snippet: The cytotoxicity of the films was estimated from the viability of L929 fibroblasts (KAC Co., Ltd., Kyoto, Japan).

Techniques: Incubation

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: ( a ) Cytotoxic activity of the 17.1-Mts1 complex (1 nM) on L929, K562, HEK 293T, and HL-60 cells in the presence of an antibody to TNFR1 (1:100, 24 h). ( b ) Cytotoxic activity of Mts1 complexes with peptides 17.1, 17.1A, and 17.1B on L929 cells (24 h). ( c ) Concentration dependence of the cytotoxic activity of the 17.1-Mts1 complex on L929 cells (24 h). n = 5 for each point (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Concentration Assay

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-Mts1 complex on L929 cells after 3 ( a ) and 24 ( c ) hours in the presence of caspase 3 and 8 inhibitors and necrostatin1. n = 5 for each point, (* p -value < 0.05). ( b ) Western blot with antibodies to caspase 3 from cell lysates 1 h after 17.1-Mts1 addition (left) and control cells (right). ( d ) Western blot with antibodies to phospho-MLKL, RIP3, and phospho-RIP3 from cell lysates 1 h after addition of 17.1-Mts1 ( left ) and control cells ( right ).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay, Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Mts1 (S100A4) and Its Peptide Demonstrate Cytotoxic Activity in Complex with Tag7 (PGLYRP1) Peptide

doi: 10.3390/ijms25126633

Figure Lengend Snippet: Cytotoxic activity of the 17.1-M7 complex on L929 cells after 3 ( a ) and 20 ( b ) hours in the presence of caspase 3 and 8 inhibitors, necrostatin1, EGTA, calpains and cathepsins inhibitors, and antioxidant ionol. n = 5 for each point, (* p -value < 0.05).

Article Snippet: Experiments were performed with the L929 mouse fibroblast cell line, which was cultured, respectively, in DMEM (Himedia Laboratories Private Limited, Maharashtra, India) with 2 mM L-glutamine, 10% FCS (Cytiva LivescienceTM, Marlborough, MA, USA), and antibiotics (penicillin and streptomycin) (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in an atmosphere containing 5% CO 2 .

Techniques: Activity Assay

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Sulfur‐Doped Carbon Dots as a Highly Selective and Sensitive Fluorescent Probe for Copper Ion Detection in Biological Systems

doi: 10.1002/smll.202410765

Figure Lengend Snippet: Fluorescence properties of S_HICA. Excitation–emission intensity spectra of a) HICA and b) S_HICA. c) Fluorescence images of HICA and S_HICA. (Fluorescence image A: Ex: 470nm, Em: 530 nm and fluorescence image B: Ex: 530nm, Em:630nm) d) Fluorescence stability assay depending on time (n = 3). e) Confocal differential interference contrast (DIC) and fluorescence images of L929 cells incubated with HICA and S_HICA.

Article Snippet: L929 cells (i.e., normal cell line of mouse connective tissue) were sourced from the Korean Cell Line Bank (KCLB) with the assigned number 10 001.

Techniques: Fluorescence, Stability Assay, Incubation

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Sulfur‐Doped Carbon Dots as a Highly Selective and Sensitive Fluorescent Probe for Copper Ion Detection in Biological Systems

doi: 10.1002/smll.202410765

Figure Lengend Snippet: Application of S_HICA to detect Cu 2+ in biological systems in vitro, ex vivo and in vivo. a) Confocal microscopy images of L929 cells treated with various concentrations of Cu 2+ (0–100 µ m ) followed by 0.1 mg mL −1 of S_HICA. b) Fluorescence intensity of S_HICA at an excitation wavelength of 495 nm and emission wavelength of 519 nm. c) Schematic of the ex vivo experiment. Fluorescence response of S_HICA to the d) Cu 2+ concentration and e) incubation time in ex vivo. f) Fluorescence images of mice injected with S_HICA before and after the administration of Cu 2 ⁺. g) Quantitative analysis of fluorescence intensity at the injection site (n = 4) (*** p < 0.001, n.s, not significant, Two‐way ANOVA with Sidak's multiple comparisons test).

Article Snippet: L929 cells (i.e., normal cell line of mouse connective tissue) were sourced from the Korean Cell Line Bank (KCLB) with the assigned number 10 001.

Techniques: In Vitro, Ex Vivo, In Vivo, Confocal Microscopy, Fluorescence, Concentration Assay, Incubation, Injection